Baltzell, K. A., Shakely, D., Hsiang, M., Kemere, J., Ali, A. S., Björkman, A., Mårtensson, A., Omar, R., Elfving, K., Msellem, M., Aydin-Schmidt, B., Rosenthal, P. J. and Greenhouse, B. (2012) Prevalence of PCR Detectable Malaria Infection among Febrile Patients with a Negative Plasmodium falciparum Specific Rapid Diagnostic Test in Zanzibar. The American journal of tropical medicine and hygiene. ISSN 1476-1645Full text not available from this repository.
We screened for malaria in 594 blood samples from febrile patients who tested negative by a Plasmodium falciparum-specific histidine-rich protein-2-based rapid diagnostic test at 12 health facilities in Zanzibar districts North A and Micheweni, from May to August 2010. Screening was with microscopy, polymerase chain reaction (PCR) targeting the cytochrome b gene (cytbPCR) of the four major human malaria species, and quantitative PCR (qPCR). The prevalence of cytbPCR-detectable malaria infection was 2% (12 of 594), including 8 P. falciparum, 3 Plasmodium malariae, and 1 Plasmodium vivax infections. Microscopy identified 4 of 8 P. falciparum infections. Parasite density as estimated by microscopy or qPCR was > 4,000 parasites/μL in 5 of 8 cytbPCR-detectable P. falciparum infections. The infections that were missed by the rapid diagnostic test represent a particular challenge in malaria elimination settings and highlight the need for more sensitive point-of-care diagnostic tools to improve case detection of all human malaria species in febrile patients.
|Keywords:||Malaria Infection;Febrile Patients;Plasmodium falciparum; Zanzibar|
|Subjects:||Malaria > Diagnosis & treatment|
|Depositing User:||Mr Joseph Madata|
|Date Deposited:||15 Jan 2013 08:17|
|Last Modified:||15 Jan 2013 08:17|
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