ehealth digital library

Digital library of
the Tanzania
health
community

Early and Efficient Detection of Mycobacterium tuberculosis in Sputum by Microscopic Observation of Broth Cultures.

Kidenya, B. R., Kabangila, R., Peck, R. N., Mshana, S. E., Webster, L. E., Koenig, S. P., Johnson, W. D. and Fitzgerald, D. W. (2013) Early and Efficient Detection of Mycobacterium tuberculosis in Sputum by Microscopic Observation of Broth Cultures. PloS one, 8 (2). ISSN 1932-6203

[img]
Preview
PDF
Kidenya(1).pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (193kB)

Abstract

Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and detecting in sputum. Sputum samples were cultured in liquid assay (micro broth culture) in microplate wells and growth was detected by microscopic observation, or in Löwenstein-Jensen (LJ) solid media where growth was detected by visual inspection for colonies. Sputum samples were collected from 321 tuberculosis (TB) suspects attending Bugando Medical Centre, in Mwanza, Tanzania, and were cultured in parallel. Pulmonary tuberculosis cases were diagnosed using the American Thoracic Society diagnostic standards. There were a total of 200 (62.3%) pulmonary tuberculosis cases. Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) ( = 0.0007). The median turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ solid culture, 9 days (interquartile range [IQR] 7-13), versus 21 days (IQR 14-28) (<0.0001). The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture. The liquid assay (micro broth culture) is an early, feasible, and inexpensive method for detection of pulmonary tuberculosis in resource limited settings.

Item Type: Article
Keywords: Mycobacterium Tuberculosis, Sputum, Microscopic Observation, Broth Cultures,Pulmonary Tuberculosis, Mwanza, Tanzania
Subjects: Tuberculosis > Diagnosis
Divisions: Catholic University of Health and Allied Sciences (CUHAS)
Depositing User: Mr Joseph Madata
Date Deposited: 30 Apr 2013 06:51
Last Modified: 30 Apr 2013 06:51
URI: http://ihi.eprints.org/id/eprint/1215

Actions (login required)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics