Ng'habi, K. R., Horton, A., Knols, B. G. J. and Lanzaro, G. C. (2007) A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes. The American journal of tropical medicine and hygiene, 77 (3). pp. 485-7. ISSN 0002-9637
485.full.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.
The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.
|Keywords:||Malaria, female Anopheles gambiae, mosquito, Polymerase Chain Reaction, Africa|
|Subjects:||Malaria > Diagnosis & treatment|
|Divisions:||Ifakara Health Institute > Biomedical|
|Depositing User:||Mr Joseph Madata|
|Date Deposited:||16 Jul 2012 09:13|
|Last Modified:||16 Aug 2012 15:57|
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