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Kinetics of Humoral and Memory B Cell Response Induced by the Plasmodium Falciparum 19-kilodalton Merozoite Surface Protein 1 in mice.

Kafuye-Mlwilo, M. Y., Mukherjee, P. and Chauhan, V. S. (2012) Kinetics of Humoral and Memory B Cell Response Induced by the Plasmodium Falciparum 19-kilodalton Merozoite Surface Protein 1 in mice. Infection and immunity, 80 (2). pp. 633-42. ISSN 1098-5522

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Abstract

The 19-kDa carboxyl-terminal fragment of the merozoite surface protein-1 (MSP-1(19)) has been shown to regulate antibody (Ab)-mediated protective immunity to blood-stage malaria infection. But the serological memory to this antigen tends to be short-lived, and little is known of the mechanisms that regulate the formation of B cell memory to MSP-1(19) antigen. We studied the formation of B cell memory response after immunization with the recombinant 19-kDa Plasmodium falciparum merozoite surface protein 1 (PfMSP-1(19)). Immunization with PfMSP-1(19) resulted in delayed increase in germinal center (GC) B cell numbers. This poor GC reaction correlated with short-lived PfMSP-1(19)-specific antibodies in serum and the short life of PfMSP-1(19)-specific plasma cells and memory B cells (MBCs) in spleen and bone marrow. PfMSP-1(19)-specific MBCs were capable of producing antigen (Ag)-specific Ab-secreting cell (ASC) responses that were short-lived following challenge immunization of the immune mice with antigen or transgenic Plasmodium berghei parasite expressing PfMSP-1(19) in place of native P. berghei MSP-1(19) at 8 weeks after the last immunization or following adoptive transfer into naive hosts. However, no protection was achieved in PfMSP-1(19) immune mice or recipient mice with PfMSP-1(19)-specific MBCs following challenge with transgenic P. berghei. Our findings suggest that PfMSP-1(19)-specific IgG production by short-lived plasma cells combined with the poor ability of the PfMSP-1(19)-induced MBCs to maintain the anamnestic IgG responses failed to contribute to protection against infection.

Item Type: Article
Keywords: Plasmodium Falciparum, Malaria, protein-1, antibody, malaria infection
Subjects: Malaria > Diagnosis & treatment
Divisions: National Institute for Medical Research
Depositing User: Mr Joseph Madata
Date Deposited: 14 May 2014 09:38
Last Modified: 14 May 2014 09:38
URI: http://ihi.eprints.org/id/eprint/1765

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