ehealth digital library

Digital library of
the Tanzania
health
community

Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania.

Talbot, E. A., Maro, I., Ferguson, K., Adams, L. V., Mtei, L., Matee, M. and von Reyn, C. F. (2012) Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania. Tuberculosis research and treatment, 2012. p. 345290. ISSN 2090-1518

[img]
Preview
PDF
Elizabeth_A._Talbot.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (489kB)

Abstract

Background. T-SPOT.TB is an interferon gamma release assay for detecting Mycobacterium tuberculosis infection. The requirement to process within 8 hours is constraining, deters use, and leads to invalid results. Addition of T Cell Xtend reagent may allow delayed processing, but has not been extensively field tested. Design. Consecutive AFB smear positive adult tuberculosis patients were prospectively recruited in Dar es Salaam, Tanzania. Patients provided a medical history, 1-3 sputum samples for culture and 1 blood sample which was transported to the laboratory under temperature-controlled conditions. After overnight storage, 25 μL of T Cell Xtend reagent was added per mL of blood, and the sample was tested using T-SPOT.TB. Results. 143 patients were enrolled: 57 patients were excluded because temperature control was not maintained, 19 patients were excluded due to red blood cell contamination, and one did not provide a sputum sample for culture. Among 66 evaluable patients, overall agreement between T-SPOT.TB and culture was 95.4% (95%CI; 87.1-99.0%) with Kappa value 0.548. Sensitivity of T-SPOT.TB when using T Cell Xtend reagent was 96.8% (95%CI; 88.8-99.6%). Conclusions. When T Cell Xtend reagent is added to specimens held overnight at recommended temperatures, T-SPOT.TB is as sensitive as the standard assay in patients with tuberculosis.

Item Type: Article
Keywords: T-SPOT,TB,Tuberculosis Infection, HIV, Dar es Salaam, Tanzania
Subjects: Tuberculosis > Diagnosis
Divisions: Muhimbili University of Health and Allied Sciences (MUHAS)
Depositing User: Mr Joseph Madata
Date Deposited: 17 Oct 2013 08:40
Last Modified: 17 Oct 2013 08:56
URI: http://ihi.eprints.org/id/eprint/1969

Actions (login required)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics