Okumu, F. O., Titus, E., Mbeyela, E., Killeen, G. F. and Moore, S. J. (2009) Limitation of using synthetic human odours to test mosquito repellents. Malaria journal, 8 (150). pp. 1-7. ISSN 1475-2875
1475-2875-8-150.pdf - Published Version
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Gold-standard tests of mosquito repellents involve exposing human volunteers to host-seeking mosquitoes, to assess the protective efficacy of the repellents. These techniques are not exposure-free and cannot be performed prior to toxicological evaluation. It is postulated that synthetic lures could provide a useful assay that mimics in-vivo conditions for use in high-throughput screening for mosquito repellents.
This paper reports on a semi-field evaluation of repellents using a synthetic blend of human derived attractants for the malaria vector, Anopheles gambiae sensu stricto Different concentrations of known repellents, N, N diethyl-3-methylbenzamide (deet) and Para-methane-3, 8, diol (PMD) were added into traps baited with the synthetic blend, and resulting changes in mosquito catches were measured.
All test concentrations of deet (0.001% to 100%) reduced the attractiveness of the synthetic blend. However, PMD was repellent only at 0.25%. Above this concentration, it significantly increased the attractiveness of the blend. There was no relationship between the repellent concentrations and the change in mosquito catches when either deet (r2 = 0.033, P = 0.302) or PMD (r2 = 0.020, P = 0.578) was used.
It is concluded that while some repellents may reduce the attractiveness of synthetic human odours, others may instead increase their attractiveness. Such inconsistencies indicate that even though the synthetic attractants may provide exposure-free and consistent test media for repellents, careful selection and multiple-repellent tests are necessary to ascertain their suitability for use in repellent screening. The synthetic odour blend tested here is not yet sufficiently refined to serve as replacement for humans in repellent testing, but may be developed further and evaluated in different formats for exposure free repellent testing purposes.
|Keywords:||mosquito repellents, human odours, toxicological evaluation, vector control, malaria prevention|
|Subjects:||Malaria > Surveillance, monitoring, evaluation
Malaria > Vector control
Malaria > Diagnosis & treatment
|Depositing User:||Mr Joseph Madata|
|Date Deposited:||06 Aug 2012 07:53|
|Last Modified:||16 Aug 2012 15:57|
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