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Understanding Laboratory Methods and Their Impact on Antimicrobial Resistance Surveillance, at Muhimbili National Hospital, Dar es Salaam, Tanzania

Nyasulu, P. , Kasubi, M. , Boniface, R. and Murray, J. (2014) Understanding Laboratory Methods and Their Impact on Antimicrobial Resistance Surveillance, at Muhimbili National Hospital, Dar es Salaam, Tanzania. Advances in Microbiology, 4. pp. 33-38. ISSN 2165-3402

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Abstract

The study sought to describe laboratory methods and blood culture procedures and their impact on antimicro-bial resistance surveillance among nosocomial bacteria. We conducted a systematic audit of blood culture pro-cedures and practices in the Department of Microbiology, Central Pathology Laboratory at Muhimbili National Hospital, between 19th and 23rd March 2012. A total of 25 - 30 blood culture specimens were received each day as an indication of low volumes of blood culturing at this site. More blood culture requests came from the neonatal unit of the hospital, and were performed manually with high culture negative specimens. The laboratory per-formed antibiotic susceptibility testing as per the CLSI guidelines. No vancomycin resistance was ever reported at this site. All blood culture results were entered into the JEEVA laboratory information system, where results could be accessed by clinicians in the wards and data could be retrieved to assess patterns of antimicrobial resis-tance. Blood culture data entry system lacked quality control checks hence numerous errors and missing data were observed. Our results support the relevance of having improved laboratory procedures and good quality blood culture since surveillance of antimicrobial resistance primarily depends on good laboratory procedures, good quality and reliable blood culture data. This would essentially minimise imprecise estimates of rates of an-timicrobial resistance at this hospital.

Item Type: Article
Keywords: Antimicrobial Resistance, Microbiology, Surveillance, Laboratory, Pathology, Muhimbili, Tanzania
Subjects: Health Systems > Surveillance, monitoring & evaluation
Health Systems > Laboratories, diagnostics, equipment
Divisions: Muhimbili University of Health and Allied Sciences (MUHAS)
Depositing User: Mr Joseph Madata
Date Deposited: 06 Feb 2014 08:45
Last Modified: 06 Feb 2014 08:47
URI: http://ihi.eprints.org/id/eprint/2364

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