Zhong, D., Temu, E. A., Guda, T., Gouagna, L., Menge, D., Pai, A., Githure, J., Beier, J. C. and Yan, G. (2006) Dynamics of gene introgression in the African malaria vector Anopheles gambiae. Genetics, 172 (4). pp. 2359-2365. ISSN 0016-6731 (In Press)
GEN17242359.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.
Anopheles gambiae is a major malaria vector in Africa and a popular model species for a variety of ecological, evolutionary, and genetic studies on vector control. Genetic manipulation of mosquito vectorial capacity is a promising new weapon for the control of malaria. However, the release of exotic transgenic mosquitoes will bring in novel alleles in addition to the parasite-inhibiting genes, which may have unknown effects on the local population. Therefore, it is necessary to develop methodologies that can be used to evaluate the spread rate of introduced genes in A. gambiae. In this study, the effects and dynamics of genetic introgression between two geographically distinct A. gambiae populations from western Kenya (Mbita) and eastern Tanzania (Ifakara) were investigated with amplified fragment length polymorphisms (AFLPs) and microsatellite markers. Microsatellites and polymorphic cDNA markers revealed a large genetic differentiation between the two populations (average F(ST) = 0.093, P < 0.001). When the two strains were crossed in random mating between the two populations, significant differences in the rate of genetic introgression were found in the mixed populations. Allele frequencies of 18 AFLP markers (64.3%) for Mbita and of 26 markers (92.9%) for Ifakara varied significantly from F5 to F20. This study provides basic information on how a mosquito release program would alter the genetic makeup of natural populations, which is critical for pilot field testing and ecological risk evaluation of transgenic mosquitoes.
|Keywords:||Genes, Malaria Vvector, Anopheles gambiae, malaria control, mosquito|
|Subjects:||Malaria > Vector control|
|Divisions:||Ifakara Health Institute > Biomedical|
|Depositing User:||Mr Joseph Madata|
|Date Deposited:||30 Jul 2012 06:16|
|Last Modified:||16 Aug 2012 15:57|
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