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Occurrence of pfatpase6 Single Nucleotide Polymorphisms Associated with Artemisinin Resistance among Field Isolates of Plasmodium falciparum in North-Eastern Tanzania

Chilongola, J., Ndaro, A., Tarimo, H., Shedrack, T., Barthazary, S., Kaaya, R., Masokoto, A., Kajeguka, D., Kavishe, R. A. and Lusingu, J. (2015) Occurrence of pfatpase6 Single Nucleotide Polymorphisms Associated with Artemisinin Resistance among Field Isolates of Plasmodium falciparum in North-Eastern Tanzania. Malaria Research and Treatment. ISSN 2090-8075

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Abstract

We aimed to determine the current prevalence of four P. falciparum candidate artemisinin resistance biomarkers L263E, E431K, A623E, and S769N in the pfatpase6 gene in a high transmission area in Tanzania in a retrospective cross sectional study using 154 archived samples collected from three previous malaria studies in 2010, 2011 and 2013. Mutations in pfatpase6 gene were detected in parasite DNA isolated from Dried Blood Spots by using PCR-RFLP. We observed overall allelic frequencies for L263E, E431K, A623E, and S769N to be 5.8% (9/154), 16.2% (25/154), 0.0% (0/154), and 3.9% (6/154). The L263E mutation was not detected in 2010 but occurred at 3.9% and 2.6% in 2011 and 2013 respectively. The L263E mutation showed a significant change of frequency between 2010 and 2011, but not between 2011 and 2013 (

Item Type: Article
Keywords: pfatpase6, Nucleotide Polymorphisms, Artemisinin Resistance, Plasmodium falciparum, Tanzania
Subjects: Malaria > Diagnosis & treatment
Divisions: Kilimanjaro Christian Medical Centre
Depositing User: Mr Joseph Madata
Date Deposited: 14 Jan 2015 09:41
Last Modified: 14 Jan 2015 09:41
URI: http://ihi.eprints.org/id/eprint/3031

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