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Mass Spectrometry Identification of Age-associated Proteins from the Malaria Mosquitoes Anopheles Gambiae s.s. and Anopheles Stephensi.

Sikulu, M. T., Monkman, J., Dave, K. A., Hastie, M. L., Dale, P. E., Kitching, R. L., Killeen, G. F., Kay, B. H., Gorman, J. J. and Hugo, L. E. (2015) Mass Spectrometry Identification of Age-associated Proteins from the Malaria Mosquitoes Anopheles Gambiae s.s. and Anopheles Stephensi. Data in brief, 4. pp. 461-7. ISSN 2352-3409

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Abstract

This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

Item Type: Article
Keywords: Malaria, Mosquitoes, nopheles Gambiae s.s., Anopheles stephensi, Mass Spectrometry Identification,
Subjects: Malaria > Vector control
Divisions: Ifakara Health Institute > Environmental
Depositing User: Mr Joseph Madata
Date Deposited: 30 Sep 2015 08:55
Last Modified: 30 Sep 2015 08:55
URI: http://ihi.eprints.org/id/eprint/3376

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