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Molecular Characterization of Diarrheagenic Bacteria Isolated from Stool of Under-five Children in Dar Es Salaam. Tanzania

Benjamin, B. E., Namkinga, L. A. and Nkwengulila, G. (2016) Molecular Characterization of Diarrheagenic Bacteria Isolated from Stool of Under-five Children in Dar Es Salaam. Tanzania. Journal of Biology and Life Science, 7 (1). pp. 71-84. ISSN 2157-6076

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Abstract

Diarrhea is a daily public health song in developing countries like Tanzania.The causative agents are theoretically known almost to everybody. However, the eradication of this killer disease for the under-fives is an enigma. This study aimed toprovide update advantages of molecular diagnostic versus conventional methods as regards to acute diarrhea, and to determine bacterialcausesof diarrhea among children aging five years and below in Dar Es Salaam, Tanzania, using multiplex PCR technique. Samples were collected fromthe under-fives from district hospitals in Dar Es Salaam city between June 2010 and February 2014. This included children admitteddue to acute and/ or chronic diarrhea. A total of 3600stool samples were analyzed, of which 1800 samples were from diarrhea cases and 1800samples from normal control cases. About 1080 (60%)of the patients recruited were aged less than 3 years and 983 (54.6%) were males. Diarrheagenic bacteria were isolated and identified using conventional stool cultures then were characterized by mPCR. Pathogenic bacteriawere detected in 67.7% of the cases and in 20% of the controls. The pathogenic bacteriamost strongly associated with diarrhea disease were diarrheagenic Escherichia coli(21.6% of cases, 6% of controls), Shigellaspp. (16.1% of cases, 5% of controls) and Salmonellae, (10.6% of cases, 3% of controls. The pathogenic bacteria weremostly from children aging from 24 months and above. Diarrheagenic bacteria play an important role in relationto childhood diarrhea aging from two years and above. Proper diagnostic methods, prevention and controlthrough fostering good hygiene and sanitation to water and food should be emphasizedespecially to oral-faecal age.

Item Type: Article
Keywords: Childhood diarrhea, Diarrheagenic bacteria, Pathogenic bacteria, Salmonellae
Subjects: Emerging and Re-emerging Infectious Diseases
Divisions: Ifakara Health Institute > Health Systems
Depositing User: Mr Joseph Madata
Date Deposited: 04 Feb 2016 07:19
Last Modified: 04 Feb 2016 07:19
URI: http://ihi.eprints.org/id/eprint/3594

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