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Direct Susceptibility Testing of Mycobacterium Tuberculosis for Pyrazinamide using the BACTEC MGIT 960 System.

Demers, A.-M., Venter, A., Friedrich, S. O., Rojas-Ponce, G., Mapamba, D., Jugheli, L., Sasamalo, M., Almeida, D., Dorasamy, A., Jentsch, U., Gibson, M., Everitt, D., Eisenach, K. D. and Diacon, A. H. (2016) Direct Susceptibility Testing of Mycobacterium Tuberculosis for Pyrazinamide using the BACTEC MGIT 960 System. Journal of clinical microbiology. ISSN 1098-660X

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Pyrazinamide is a key antituberc ulosis drug, yet no rapid susceptibility test is commercially available. PZA drug susceptibility testing (DST) was performed directly on the sputum from 327 patients and compared with the indirect method using the BACTEC MGIT 960 system in the context of patient screening for participation in a drug trial. Compared to standard indirect PZA DST, direct DST was only successful in 59% of cases, but results obtained were highly accurate and available faster. Agreement between the direct and indirect method varied from 90 to 100% in each laboratory. The median times for obtaining PZA results from the time the specimen was collected ranged from 11 to 16 days for the direct and 18 to 95 days for the indirect across laboratories. The direct method is accurate and reproducible across laboratories. It can be expected to accelerate results in more than 50% of cases but it cannot replace indirect DST for PZA. Phenotypic methods remain the gold standard for DST in drug trials. If future studies can optimize the method to decrease the number of uninterpretable results, direct MGIT DST could be the new phenotypic DST standard for clinical trials providing more rapid detection of resistance to new drugs in experimental regimens.

Item Type: Article
Keywords: Pyrazinamide, Tuberculosis, Drug resistance testing, Liquid culture
Subjects: Tuberculosis > Diagnosis
Tuberculosis > Treatment
Tuberculosis > Drug-resistant TB
Divisions: Ifakara Health Institute > Biomedical
Depositing User: Mr Joseph Madata
Date Deposited: 03 Mar 2016 07:37
Last Modified: 03 Mar 2016 07:37

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