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Quantification of Plasmodium Falciparum Histidine-rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients.

Mikita, K., Thakur, K., Anstey, N. M., Piera, K. A., Pardo, C. A., Weinberg, J. B., Mukemba, J., Florence, S., Mwaikambo, E. D., Granger, D. L. and Sullivan, D. J. (2014) Quantification of Plasmodium Falciparum Histidine-rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients. The American journal of tropical medicine and hygiene, 91 (3). pp. 486-92. ISSN 1476-1645

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Abstract

A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.

Item Type: Article
Keywords: Plasmodium falciparum histidine-rich protein-2 (PfHRP-2), Cerebrospinal Fluid, Cerebral Malaria Patients
Subjects: Malaria > Diagnosis & treatment
Divisions: Other
Depositing User: Mr Joseph Madata
Date Deposited: 30 Jun 2016 07:18
Last Modified: 30 Jun 2016 07:18
URI: http://ihi.eprints.org/id/eprint/3812

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