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RTS,S/AS01E Malaria Vaccine Induces Memory and Polyfunctional T Cell Responses in a Pediatric African Phase III Trial.

Moncunill, G., De Rosa, S. C., Ayestaran, A., Nhabomba, A. J., Mpina, M., Cohen, K. W., Jairoce, C., Rutishauser, T., Campo, J. J., Harezlak, J., Sanz, H., Díez-Padrisa, N., Williams, N. A., Morris, D., Aponte, J. J., Valim, C., Daubenberger, C., Dobaño, C. and McElrath, M. J. (2017) RTS,S/AS01E Malaria Vaccine Induces Memory and Polyfunctional T Cell Responses in a Pediatric African Phase III Trial. Frontiers in immunology, 8. p. 1008. ISSN 1664-3224

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Comprehensive assessment of cellular responses to the RTS,S/AS01E vaccine is needed to understand potential correlates and ultimately mechanisms of protection against malaria disease. Cellular responses recognizing the RTS,S/AS01E-containing circumsporozoite protein (CSP) and Hepatitis B surface antigen (HBsAg) were assessed before and 1 month after primary vaccination by intracellular cytokine staining and 16-color flow cytometry in 105 RTS,S/AS01-vaccinated and 74 rabies-vaccinated participants (controls) in a pediatric phase III trial in Africa. RTS,S/AS01E-vaccinated children had significantly higher frequencies of CSP- and HBsAg-specific CD4+ T cells producing IL-2, TNF-α, and CD40L and HBsAg-specific CD4+ T producing IFN-γ and IL-17 than baseline and the control group. Vaccine-induced responses were identified in both central and effector memory (EM) compartments. EM CD4+ T cells expressing IL-4 and IL-21 were detected recognizing both vaccine antigens. Consistently higher response rates to both antigens in RTS,S/AS01E-vaccinated than comparator-vaccinated children were observed. RTS,S/AS01E induced polyfunctional CSP- and HBsAg-specific CD4+ T cells, with a greater degree of polyfunctionality in HBsAg responses. In conclusion, RTS,S/AS01E vaccine induces T cells of higher functional heterogeneity and polyfunctionality than previously characterized. Responses detected in memory CD4+ T cell compartments may provide correlates of RTS,S/AS01-induced immunity and duration of protection in future correlates of immunity studies.

Item Type: Article
Keywords: malaria, Plasmodium falciparum, Vaccine, Cellular immune responses, T cells, Intracellular cytokine staining, Flow cytometry
Subjects: Malaria > Vaccines
Divisions: Ifakara Health Institute > Interventions
Depositing User: Mr Joseph Madata
Date Deposited: 13 Jan 2018 15:06
Last Modified: 13 Jan 2018 15:06

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